Bapx1 acts as a negative regulator of chondrocyte maturation. The constitutive RelA activation mediated by Bapx1 controls chondrocyte viability.1) Provot, S., (2006) Development 133 (4), 651-662.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Bapx1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
Bapx1 antibody can be used for detection of Bapx1 by ELISA at 1:62500. Bapx1 antibody can be used for detection of Bapx1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Jurkat 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
Bapx1 antibody can be used for detection of Bapx1 by ELISA at 1:62500. Bapx1 antibody can be used for detection of Bapx1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store Bapx1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.