Application
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.| Product Name | BCAR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.1) van Agthoven T, van Agthoven TL, Dekker A, et al. Identification of BCAR3 by a random search for genes involved in antiestrogen resistnace of human breast cancer cells. EMBO J. 1998; 17:2799-808. |
| Immunogen | BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3. The immunogen is located within amino acids 30 - 80 of BCAR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAR3 |
|---|---|
| Alternative Names | BCAR3 Antibody: NSP2 SH2D3B NSP2 UNQ271/PRO308 Breast cancer anti-estrogen resistance protein 3 Novel SH2-containing protein 2 |
| Molecular Weight(MW) | 91 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins. |
Application
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCAR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BCAR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
