Application
Immunofluorescence of BCAS1 in Human Breast Carcinoma cells with BCAS1 antibody at 20 μg/mL.Application
Immunohistochemistry of BCAS1 in human breast carcinoma with BCAS1 antibody at 5 μg/mL.Application
Western blot analysis of BCAS1 in human lung tissue lysate with BCAS1 antibody at 1 μg/mL.| Product Name | BCAS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCAS1 Antibody: BCAS1 was identified through positional cloning and was found to be overexpressed in most but not all breast cancer cells lines. Little is known about this protein; BCAS1 is cytoplasmically localized and is thought to form homodimers through coiled-coil structures. High levels of BCAS1 mRNA are seen in brain and prostate tissues, with lower amounts observed in colon, intestine and testis. Cells engineered to overexpress BCAS1 did not lose anchorage-dependent growth or increase their rate of growth, suggesting that BCAS1 is not a prototypical oncogene. The BCAS1 gene was also found to be amplified in other carcinomas such as pancreatic carcinoma, suggesting that BCAS1 may play in important role in the control of cell proliferation.1) Collins C, Rommens JM, Kowbel D, et al. Positional cloning of ZNF217 and NABC1: genes amplified at 20q13.2 and overexpressed in breast carcinoma. Proc. Natl. Acad. Sci. USA1998; 95:8703-8. |
| Immunogen | BCAS1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human BCAS1. The immunogen is located within amino acids 460 - 510 of BCAS1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BCAS1 antibody can be used for detection of BCAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAS1 |
|---|---|
| Alternative Names | BCAS1 Antibody: AIBC1 NABC1 AIBC1 Breast carcinoma-amplified sequence 1 Amplified and overexpressed in breast cancer |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat: (75%) |
Application
Immunofluorescence of BCAS1 in Human Breast Carcinoma cells with BCAS1 antibody at 20 μg/mL.
Application
Immunohistochemistry of BCAS1 in human breast carcinoma with BCAS1 antibody at 5 μg/mL.
Application
Western blot analysis of BCAS1 in human lung tissue lysate with BCAS1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | BCAS1 antibody can be used for detection of BCAS1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCAS1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BCAS1 Antibody is supplied in PBS containing 0.02% sodium azide. |
