Application
Immunohistochemistry of BCAS3 in human breast carcinoma with BCAS3 antibody at 5 μg/mL.Application
Immunofluorescence of BCAS3 in human breast carcinoma tissue with BCAS3 antibody at 20 μg/mL.Application
Western blot analysis of BCAS3 in rat brain tissue lysate with BCAS3 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | BCAS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCAS3 Antibody: The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers. Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder. BCAS3 is thought to be an estrogen receptor (ER)-alpha co-activator that acts through PELP1, another ER-alpha that in turn activates BCAS3 expression. This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer. BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.1) Barlund M, Monni O, Weaver JD, et al. Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. Genes Chromosomes Cancer2002; 35:311-7. |
| Immunogen | BCAS3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human BCAS3. The immunogen is located within amino acids 50 - 100 of BCAS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BCAS3 antibody can be used for detection of BCAS3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAS3 |
|---|---|
| Alternative Names | BCAS3 Antibody: MAAB GAOB1 Breast carcinoma-amplified sequence 3 |
Application
Immunohistochemistry of BCAS3 in human breast carcinoma with BCAS3 antibody at 5 μg/mL.
Application
Immunofluorescence of BCAS3 in human breast carcinoma tissue with BCAS3 antibody at 20 μg/mL.
Application
Western blot analysis of BCAS3 in rat brain tissue lysate with BCAS3 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | BCAS3 antibody can be used for detection of BCAS3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BCAS3 Antibody is supplied in PBS containing 0.02% sodium azide. |
