Application
Immunohistochemistry of BCAS4 in human breast carcinoma with BCAS4 antibody at 5 μg/mL.Application
Immunofluorescence of BCAS4 in human breast carcinoma tissue with BCAS4 antibody at 20 μg/mL.Application
Western blot analysis of BCAS4 in 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BCAS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCAS4 Antibody: The Breast carcinoma amplified sequence (BCAS4) gene is localized to 20q13, a region that is often amplified in breast cancers. It was isolated by the cloning of a highly overexpressed 1.3 kb transcript from the fusion of the last two exons of the BCAS3 and BCAS4 genes. This BCAS3-BCAS4 fusion transcript is only found in the MCF7 cell line, but the BCAS4 gene is also overexpressed in nine of thirteen breast cancer cell lines. The role of BCAS4 is unknown but its mRNA is highly expressed in thymus, spleen, kidney, brain, and placenta, suggesting BCAS4 plays other roles in multiple tissues.1) Barlund M, Monni O, Weaver JD, et al. Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. Genes Chromosomes Cancer2002; 35:311-7. |
| Immunogen | BCAS4 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human BCAS4. The immunogen is located within the first 50 amino acids of BCAS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BCAS4 antibody can be used for detection of BCAS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAS4 |
|---|---|
| Alternative Names | BCAS4 Antibody: CNOL Breast carcinoma-amplified sequence 4 |
Application
Immunohistochemistry of BCAS4 in human breast carcinoma with BCAS4 antibody at 5 μg/mL.
Application
Immunofluorescence of BCAS4 in human breast carcinoma tissue with BCAS4 antibody at 20 μg/mL.
Application
Western blot analysis of BCAS4 in 3T3 cell lysate with BCAS4 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | BCAS4 antibody can be used for detection of BCAS4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCAS4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BCAS4 Antibody is supplied in PBS containing 0.02% sodium azide. |
