Application
Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | BCDIN3D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | O-methyltransferase that specifically dimethylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre- miRNAs.1) Ota T.,et al.Nat. Genet. 36:40-45(2004). |
| Immunogen | This BCDIN3D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from human BCDIN3D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000-2000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCDIN3D |
|---|---|
| Alternative Names | Pre-miRNA 5'-monophosphate methyltransferase 2.1.1.- BCDIN3 domain-containing protein BCDIN3D |
| Molecular Weight(MW) | 33 kDa |
Application
Western Blot at 1:1000-2000 dilution Lane 1: A431 whole cell lysate Lane 2: human cerebellum lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000-2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
