Application
Immunofluorescence of Bcl-10 in Raji cells with Bcl-10 antibody at 10 μg/mL.Application
Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/mL.Application
Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.| Product Name | Bcl-10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-10 Antibody: Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-κB activation. Bcl10 interacts with caspase-9 and enhances pro-caspase-9 processing and induces apoptosis through caspase-9 activation. Bcl10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy. Bcl10 is expressed in many human and murine tissues and cell lines.1) Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell 1999;96(1):35-45 |
| Immunogen | Bcl10 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bcl10. The immunogen is located within the first 50 amino acids of Bcl-10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Bcl-10 antibody can be used for detection of BCL10 by Western blot at 0.5 μg/mL. dilution. An approximately 31 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL10 |
|---|---|
| Alternative Names | Bcl-10 Antibody: CLAP mE10 CIPER c-E10 CARMEN CLAP CARD-containing molecule enhancing NF-kappa-B Bcl-10 |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
Application
Immunofluorescence of Bcl-10 in Raji cells with Bcl-10 antibody at 10 μg/mL.
Application
Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/mL.
Application
Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Bcl-10 antibody can be used for detection of BCL10 by Western blot at 0.5 μg/mL. dilution. An approximately 31 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-10 Antibody is supplied in PBS containing 0.02% sodium azide. |
