Application
Immunohistochemical analysis of paraffin - embedded liver carcinoma showing cytoplasmic localization using BCL10 antibody with DAB staining.Application
Western blot analysis using BCL10 monoclonal antibody against truncated BCL10 recombinant protein and MCF - 7 cell lysate.| Product Name | BCL10 Antibody [4F8E8H8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | B-cell CLL/lymphoma 10, also known as CLAP, Me10, CIPER, c-E10, CARMEN. Entrez Protein NP_003912. It is a protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-κB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-κB signaling. Bcl-10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-κB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.1) Willis, T.G., et al. (1999) Cell. 96, 35-45. |
| Immunogen | Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| IHC(P):Dilution 1:1,000 - 1:2,000 IHC(F):Dilution 1:1,000 - 1:2,000 Western blot:Dilution 1:2,000 - 1:5,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL10 |
|---|---|
| Alternative Names | CIPER CLAP CARD-containing molecule enhancing NF-kappa-B Bcl-10 mE10 CIPER c-E10 CARMEN |
Application
Immunohistochemical analysis of paraffin - embedded liver carcinoma showing cytoplasmic localization using BCL10 antibody with DAB staining.
Application
Western blot analysis using BCL10 monoclonal antibody against truncated BCL10 recombinant protein and MCF - 7 cell lysate.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | IHC(P):Dilution 1:1,000 - 1:2,000 IHC(F):Dilution 1:1,000 - 1:2,000 Western blot:Dilution 1:2,000 - 1:5,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
| Form | Liquid |
|---|---|
| Storage Instructions | BCL-10 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
