Application
Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 μg/mL.Application
Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 μg/mL.Application
Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 μg/mL.| Product Name | Bcl-rambo Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-rambo Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo. The immunogen is located within amino acids 280 - 330 of Bcl-rambo. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L13 |
|---|---|
| Alternative Names | Bcl-rambo Antibody: MIL1 BCL-RAMBO Bcl2-L-13 MIL1 CD003 Bcl-2-like protein 13 Bcl-rambo |
| Sequence Similarities | 3) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunohistochemistry of Bcl-rambo in human heart tissue with Bcl-rambo antibody at 10 μg/mL.
Application
Immunofluorescence of Bcl-rambo in Human Heart cells with Bcl-rambo antibody at 20 μg/mL.
Application
Western blot analysis of Bcl-rambo in K562 cell lysate with Bcl-rambo antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl-rambo antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide. |
