Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BCLW antibody detected the BCLW protein (arrow).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of Bcl-w3 using rabbit polyclonal Bcl-w BH3 Domain Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2).Application
Western blot analysis of anti-Bcl-w BH3 domain Pab in HL-60 cell lysate. Bcl-w BH3 domain was detected using purified Pab.| Product Name | Bcl-w Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl-w is a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators. Expression of this gene in cells has been shown to contribute to reduced cell apoptosis under cytotoxic conditions. Studies of the related gene in mice indicated a role in the survival of NGF- and BDNF-dependent neurons. Mutation and knockout studies of the mouse gene demonstrated an essential role in adult spermatogenesis.1) Denisov, A.Y., et al., J. Biol. Chem. 278(23):21124-21128 (2003). |
| Immunogen | This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-59 amino acids from human Bcl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | BCL2L2 |
|---|---|
| Alternative Names | Bcl-2-like protein 2 Bcl2-L-2 Apoptosis regulator Bcl-W BCL2L2 BCLW KIAA0271 |
| Molecular Weight(MW) | 21 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the BCLW antibody detected the BCLW protein (arrow).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Bcl-w BH3 Domain Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of Bcl-w3 using rabbit polyclonal Bcl-w BH3 Domain Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BCL2L2 gene (Lane 2).
Application
Western blot analysis of anti-Bcl-w BH3 domain Pab in HL-60 cell lysate. Bcl-w BH3 domain was detected using purified Pab.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
