Application
Immunocytochemistry of Bcl9L in HeLa cells with Bcl9L antibody at 10 μg/mL.Application
Immunofluorescence of Bcl9L in human breast tissue with Bcl9L antibody at 20 μg/ml.Application
Immunohistochemistry of Bcl9L in human breast tissue with Bcl9L antibody at 5 μg/ml.Application
Western blot analysis of Bcl9L in HeLa cell lysate with Bcl9L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | Bcl9L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bcl9L Antibody: Bcl9L, a homolog of Bcl9, was initially identified through a bioinformatics screening. It is expressed in fetal brain, adult lung, eye and prostate, in addition to several types of tumors including pancreatic and prostate cancers. Bcl9L has been shown to interact with beta-catenin, a target of the Wnt signaling pathway, and is required for enhanced beta-catenin-T-cell factor (TCF)-mediated transcription in colorectal tumor cells, possibly by translocating beta-catenin to the nucleus. Other studies have indicated that Bcl9L expression correlates with high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers, suggesting that activity may occur in other types of cancer. Bcl9L has also been shown to be critical for Wnt-mediate regulation of stem cell traits in colon epithelium and adenocarcinomas which are associated with tumor invasion, metastasis, and resistance to therapy.1) Katoh M and Katoh M. Identification and characterization of human BCL9L gene and mouse Bcl9l gene in silico. Int. J. Mol. Med. 2003; 12:643-9. |
| Immunogen | Bcl9L antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human Bcl9L. The immunogen is located within amino acids 20 - 70 of Bcl9L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL9L |
|---|---|
| Alternative Names | Bcl9L Antibody: BCL9-2 DLNB11 Protein BCL9-2 B-cell lymphoma 9-like protein |
| Molecular Weight(MW) | Predicted: 165 kDa Observed: 195 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | Bcl9L antibody is predicted to not cross-react with other Bcl family members. At least four isoforms of Bcl9L are known to exist; this antibody will detect all four. |
Application
Immunocytochemistry of Bcl9L in HeLa cells with Bcl9L antibody at 10 μg/mL.
Application
Immunofluorescence of Bcl9L in human breast tissue with Bcl9L antibody at 20 μg/ml.
Application
Immunohistochemistry of Bcl9L in human breast tissue with Bcl9L antibody at 5 μg/ml.
Application
Western blot analysis of Bcl9L in HeLa cell lysate with Bcl9L antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Bcl9L antibody can be used for detection of Bcl9L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bcl9L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bcl9L Antibody is supplied in PBS containing 0.02% sodium azide. |
