Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
BCOR Antibody (Center S1122) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCOR Antibody (Center S1122) for immunohistochemistry.Application
Western blot analysis of BCOR Antibody (Center S1122) in 293 cell line lysates (35ug/lane)Application
Western blot analysis of BCOR Antibody (Center S1122) in mouse stomach tissue lysates (35ug/lane)| Product Name | BCOR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCOR was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs.1) Ghetu,A.F., Mol. Cell 29 (3), 384-391 (2008) |
| Immunogen | This BCOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1107-1137 amino acids from the Central region of human BCOR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | BCOR |
|---|---|
| Alternative Names | BCL-6 corepressor BCoR BCOR KIAA1575 |
| Molecular Weight(MW) | 192 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
BCOR Antibody (Center S1122) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCOR Antibody (Center S1122) for immunohistochemistry.
Application
Western blot analysis of BCOR Antibody (Center S1122) in 293 cell line lysates (35ug/lane)
Application
Western blot analysis of BCOR Antibody (Center S1122) in mouse stomach tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
