Application
Western Blot at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | beta Tubulin |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.1) Wang D.,et al.J. Cell Biol. 103:1903-1910(1986). |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tubb5 |
|---|---|
| Alternative Names | Tubulin beta-5 chain Tubb5 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
