Application
Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10 μg/mL.Application
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 10 μg/mL.| Product Name | Beta-actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.1) Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909. |
| Immunogen | Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin. The immunogen is located within the last 50 amino acids of Beta-actin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTB |
|---|---|
| Alternative Names | Beta-actin Antibody: BRWS1 PS1TP5BP1 Actin cytoplasmic 1 Beta-actin b-actin |
| Molecular Weight(MW) | Predicted: 41 kDa Observed: 41 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10 μg/mL.
Application
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Beta-actin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide. |
