Application
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/mL.Application
Western blot analysis of beta-Actin in A431, Daudi, HepG2, HL60, Jurkat, Human brain, Mouse lung, and Chicken liver lysate with beta-Actin antibody at 1 μg/mL.Application
Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 μg/mL.| Product Name | beta-Actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | beta-Actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging.The beta-actin-antibody can be used for the detection of the actin isoforms of b-actin.Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.1) Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909. |
| Immunogen | b-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human b-actin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTB |
|---|---|
| Alternative Names | beta-Actin Antibody: Beta actin b actin |
Application
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/mL.
Application
Western blot analysis of beta-Actin in A431, Daudi, HepG2, HL60, Jurkat, Human brain, Mouse lung, and Chicken liver lysate with beta-Actin antibody at 1 μg/mL.
Application
Immunofluorescence of beta-Actin in Hela cells with beta-Actin antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | b-actin antibody can be used for the detection of b-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | beta-Actin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | beta-Actin Antibody is supplied in PBS containing 0.02% sodium azide. |
