| Product Name | beta-Pix Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | beta-PIX acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. It interacts with PAK kinases through the SH3 domain. It may function as a positive regulator of apoptosis and is involved in cell migration. It is expressed with high levels in the central nervous system. |
| Immunogen | Recombinant beta-Pix SH3 domain. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ELISA: (direct and indirect: 1:5,000-1:10,000). Immunocytochemistry: (1:1,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | Arhgef7 |
|---|---|
| Alternative Names | Rho Guanine Nucleotide Exchange Factor 7 PAK-interacting Exchange Factor beta p85SPR COOL-1 |
| Tissue Specificity | Recognizes the multiple splice variants of human, mouse and rat beta-Pix a, b, bL, c and d-isoforms. Cross-reacts with human and rat beta-Pix. |
| Application Notes | ELISA: (direct and indirect: 1:5,000-1:10,000). Immunocytochemistry: (1:1,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
