Application
BF Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35ug/lane)| Product Name | BF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BF is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.1) Baatz,H., Klin Monatsbl Augenheilkd 226 (8), 654-658 (2009) |
| Immunogen | This BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-422 amino acids from the Central region of human BF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CFB |
|---|---|
| Alternative Names | Complement factor B C3/C5 convertase Glycine-rich beta glycoprotein GBG PBF2 Properdin factor B Complement factor B Ba fragment Complement factor B Bb fragment CFB BF BFD |
| Molecular Weight(MW) | 86 kDa |
Application
BF Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of BF Antibody in HepG2 cell line and mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
