Application
Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.Application
Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 μg/mL.Application
Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide| Product Name | BHLHA15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).1) Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13. |
| Immunogen | BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BHLHA15 |
|---|---|
| Alternative Names | BHLHA15 Antibody: MIST1 BHLHB8 MIST1 Class A basic helix-loop-helix protein 15 Class B basic helix-loop-helix protein 8 bHLHa15 |
| Molecular Weight(MW) | Predicted: 21 kDa Observed: 24 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | BHLHA15 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.
Application
Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 μg/mL.
Application
Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BHLHA15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM272469