Application
Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BHLHA15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BHLHA15 Antibody: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).1) Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13. |
| Immunogen | BHLHA15 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BHLHA15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BHLHA15 antibody can be used for Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BHLHA15 |
|---|---|
| Alternative Names | BHLHA15 Antibody: FB22 HM89 LAP3 LCR1 NPYR WHIM CD184 LESTR NPY3R NPYRL HSY3RR NPYY3R D2S201E |
| Molecular Weight(MW) | Predicted: 21 kDa Observed: 22 kDa |
Application
Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | BHLHA15 antibody can be used for Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BHLHA15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BHLHA15 Antibody is supplied in PBS containing 0.02% sodium azide. |
