Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of BHLHB5 Antibody in HL-60 cell line lysates (35ug/lane)Application
BHLHB5 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | BHLHB5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BHLHB5 inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. BHLHB5 despite the presence of an intact basic domain, does not bind to DNA.1) Stevens, J.D., et al. Differentiation 76(9):1006-1022(2008) |
| Immunogen | This BHLHB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human BHLHB5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BHLHE22 |
|---|---|
| Alternative Names | Class E basic helix-loop-helix protein 22 bHLHe22 Class B basic helix-loop-helix protein 5 bHLHb5 Trinucleotide repeat-containing gene 20 protein BHLHE22 BHLHB5 TNRC20 |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of BHLHB5 Antibody in HL-60 cell line lysates (35ug/lane)
Application
BHLHB5 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
