Application
Antibody used in WB on Human 293T at 1 ug/ml.Application
Antibody used in WB on Human Fetal Liver at 2.5 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Rat liver at: 1:2000.Application
Antibody used in WB on Rat liver at: 1:1000.| Product Name | BHMT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst (e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst (e)inemia,but such a defect has not yet been observed.1) Castro, C., (2004) Biochemistry 43 (18), 5341-5351. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BHMT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BHMT |
|---|---|
| Alternative Names | BHMT BHMT1 HEL-S-61p |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human 293T at 1 ug/ml.
Application
Antibody used in WB on Human Fetal Liver at 2.5 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Rat liver at: 1:2000.
Application
Antibody used in WB on Rat liver at: 1:1000.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BHMT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
