BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst (e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst (e)inemia,but such a defect has not yet been observed.1) Castro, C., (2004) Biochemistry 43 (18), 5341-5351.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BHMT.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Fetal Liver at 1.25 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Rat liver at: 1:2000.
Application
Antibody used in WB on Rat liver at: 1:1000.
Positive Control
1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Application Notes
BHMT antibody can be used for detection of BHMT by ELISA at 1:312500. BHMT antibody can be used for detection of BHMT by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store BHMT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt