Application
Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 μg/mL.Application
Immunofluorescence of BICD1 in Daudi cells with BICD1 antibody at 20 μg/mL.Application
Western blot analysis of BICD1 in Daudi cell lysate with BICD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | BICD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BICD1 Antibody: BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells.1) Baens M and Marynen P. A human homologue (BICD1) of the drosophila bicaudal-D gene. Genomics1997; 45:601-6. |
| Immunogen | BICD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BICD1. The immunogen is located within the last 50 amino acids of BICD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| BICD1 antibody can be used for detection of BICD1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BICD1 |
|---|---|
| Alternative Names | BICD1 Antibody: BICD Protein bicaudal D homolog 1 Bic-D 1 |
| Tissue Specificity | At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform. |
Application
Immunocytochemistry of BICD1 in Daudi cells with BICD1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BICD1 in Daudi cells with BICD1 antibody at 20 μg/mL.
Application
Western blot analysis of BICD1 in Daudi cell lysate with BICD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | BICD1 antibody can be used for detection of BICD1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BICD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BICD1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM272486