Application
Western blot analysis of BICD2 in 293 cell lysate with BICD2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BICD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BICD2 Antibody: BICD2 is the second human homolog discovered to the Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. Similar to the highly homologous protein BICD1, BICD2 can bind to dynein-dynactin complex, primarily through the dynamitin subunit of dynactin. The C-terminus of BICD2 targets the protein to the Golgi complex while the N-terminal domain of BICD2 co-immunoprecipitates with cytoplasmic dynein, suggesting BICD2 plays a role in the dynein-dynactin interaction on the surface of membranous organelles. Mice engineered to overexpress the BICD2 amino terminal domain in neurons developed amyotrophic lateral sclerosis (ALS)-like features such as Golgi fragmentation, neurofilament swelling in proximal axons, etc., suggesting that impaired dynein/dynactin function may explain some of the pathological features observed in ALS patients.1) Holland PM, Milne A, Garka K, et al. Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2. J. Biol. Chem.2002; 277:16229-40. |
| Immunogen | BICD2 antibody was raised against a 14 amino acid synthetic peptide from near the center of human BICD2. The immunogen is located within amino acids 300 - 350 of BICD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BICD2 antibody can be used for detection of BICD2 by Western blot at 1 - 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BICD2 |
|---|---|
| Alternative Names | BICD2 Antibody: SMALED2 bA526D8.1 KIAA0699 Protein bicaudal D homolog 2 Bic-D 2 |
| Molecular Weight(MW) | Predicted: 94 kDa Observed: 100 kDa |
| Tissue Specificity | This BICD2 antibody will not cross-react with BICD1. |
Application
Western blot analysis of BICD2 in 293 cell lysate with BICD2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | BICD2 antibody can be used for detection of BICD2 by Western blot at 1 - 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BICD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BICD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
