Application
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 μg/mL.Application
Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 μg/mL.Application
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Bid Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bid Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals, exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein. The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bid antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Bid. The immunogen is located within amino acids 120 - 170 of Bid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BID |
|---|---|
| Alternative Names | Bid Antibody: FP497 BH3-interacting domain death agonist p22 BID BID |
Application
Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 μg/mL.
Application
Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 μg/mL.
Application
Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bid antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bid Antibody is supplied in PBS containing 0.02% sodium azide. |
