Application
Western blot analysis of Bid in A549 cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | Bid Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bid Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals, exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein. The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Bid antibody was raised against a peptide corresponding to 14 amino acids near the middle of human Bid. The immunogen is located within amino acids 100 - 150 of Bid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BID |
|---|---|
| Alternative Names | Bid Antibody: FP497 BH3-interacting domain death agonist p22 BID BID |
Application
Western blot analysis of Bid in A549 cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bid antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bid Antibody is supplied in PBS containing 0.02% sodium azide. |
