Application
Immunocytochemistry of BIF in HeLa with BIF antibody at 10 μg/mL.Application
Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bif Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bif Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | BIF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BIF. The immunogen is located within the last 50 amino acids of Bif. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SH3GLB1 |
|---|---|
| Alternative Names | Bif Antibody: Bif-1 CGI-61 PPP1R70 dJ612B15.2 KIAA0491 Endophilin-B1 Bax-interacting factor 1 Bif-1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of BIF in HeLa with BIF antibody at 10 μg/mL.
Application
Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bif antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bif Antibody is supplied in PBS containing 0.02% sodium azide. |
