Application
Immunohistochemistry staining of SH3GLB1 in placenta tissue using SH3GLB1 monoclonal Antibody.| Product Name | SH3GLB1 Antibody [30A882.1.1] |
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| Antibody Type | Primary Antibodies |
| Product description | The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad. Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback et al (product citation 2) have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta. |
| Immunogen | A full length human Bif-1 recombinant protein. |
| Clonality | Monoclonal |
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| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| SH3GLB1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 2.5 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SH3GLB1 |
|---|---|
| Alternative Names | SH3GLB1 CGI-61 Endophilin B1 Endophilin-B1 PPP1R70 Bax-interacting factor 1 Bif-1 DJ612B15.2 KIAA0491 SH3-containing protein SH3GLB1 |
Application
Immunohistochemistry staining of SH3GLB1 in placenta tissue using SH3GLB1 monoclonal Antibody.| Application Notes | SH3GLB1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 2.5 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | SH3GLB1 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide |
