Application
Immunocyochemistry of BIM in K562 cells with Bim antibody at 10 μg/mL.Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.Application
Western blot analysis of Bim in K562 cell lysates with Bim antibody (IN2) at (A) 2.5 and (B) 5 μg/mL.| Product Name | Bim Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.1) O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395. |
| Immunogen | Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and rat. The immunogen is located within amino acids 80 - 130 of Bim. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Bim antibody can be used for detection of Bim by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L11 |
|---|---|
| Alternative Names | Bim Antibody: BAM BIM BOD Bcl-2-like protein 11 Bcl2-interacting mediator of cell death Bcl2-L-11 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocyochemistry of BIM in K562 cells with Bim antibody at 10 μg/mL.
Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.
Application
Western blot analysis of Bim in K562 cell lysates with Bim antibody (IN2) at (A) 2.5 and (B) 5 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Bim antibody can be used for detection of Bim by Western blot at 2.5 to 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bim antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bim Antibody is supplied in PBS containing 0.02% sodium azide. |
