Application
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.Application
Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.| Product Name | Bim Antibody [1C2H4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.1) O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395. |
| Immunogen | Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAICCIFWB |
| Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCL2L11 |
|---|---|
| Alternative Names | Bim Antibody [1C2H4] : BAM BIM BOD |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.
Application
Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 95-107 - Bim Recombinant Protein |
|---|---|
| Application Notes | Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bim monoclonal antibody can be stored at -20˚C, stable for one year. |
| Storage Buffer | Bim Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
