Application
A. Western blot analysis on LnCaP cell lysate using anti-PSMA RabMAb (cat. # ) dilution 1:5000.| Product Name | PSMA antibody [EP3253], N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP3253] to PSMAPSMA (Prostate-specific membrane antigen, Glutamate carboxypeptidase 2) is a membrane peptidase expressed in the prostate, central and peripheral nervous system, kidney, and small intestine . PSMA has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. It is required in the intestine for the uptake of folate, and in the brain it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide N-acetylaspartylglutamate (NAAG), thereby releasing glutamate . In the central nervous system, it is responsible for the cleavage of N-acetyl- L-aspartyl-L-glutamate yielding free glutamate in the synaptic cleft, and is implicated in various pathologic conditions associated with glutamate excitotoxicity . PSMA also has abundant and restricted expression on the surface of prostate carcinomas and the neovasculature of most other solid tumors, making it a target for cancer immunotherapy . |
| Immunogen | A synthetic peptide corresponding to residues near the N-terminus of human PSMA was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP3253 |
| Host Species | Rabbit |
| Tested Applications | FACSIPWB |
| For WB: Use at a dilution of 1:1000 - 5000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | FOLH, GCP2, FOLH1, FGCP, PSM, NAALAdase, mGCP, GCPII, PSMA, NAALAD1 |
|---|---|
| Target | PSMA |
| Molecular Weight(MW) | 110 (note) |
Application
A. Western blot analysis on LnCaP cell lysate using anti-PSMA RabMAb (cat. # ) dilution 1:5000.| Application Notes | For WB: Use at a dilution of 1:1000 - 5000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
