Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma showing membrane location using BLK antibody with DAB staining.Application
Immunohistochemical analysis of paraffin - embedded human breast tissue showing membrane location using BLK antibody with DAB staining.| Product Name | BLK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B lymphocyte kinaseMODY11 |
| Product description | BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56 kDa), belongs to the Src non-receptor tyrosine kinases family.Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.1) Theresa Tretter, Ashley E. Ross, Dominic I. Dordai. J. Exp. Med., Dec 2003; 198: 1863. |
| Immunogen | Ni-NTA purified truncated recombinant BLK expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P)1:500 - 1:2,000 IHC(F)1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | BLK |
|---|---|
| Alternative Names | B lymphocyte kinaseMODY11 |
Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma showing membrane location using BLK antibody with DAB staining.
Application
Immunohistochemical analysis of paraffin - embedded human breast tissue showing membrane location using BLK antibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P)1:500 - 1:2,000 IHC(F)1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | BLK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
