Application
Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | BLNK (phospho Tyr96) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling.1) Wang X, et al. (2012)J Biol Chem 287, 11037-48. Patterson HC, et al. (2006)Immunity 25, 55-65 . Chiu CW, et al. (2002)EMBO J 21, 6461-72. |
| Immunogen | BLNK (phospho Tyr96) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 96 (D-S-Y (p)-E-P) derived from Human BLNK . |
| Modification | p-Tyr96 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BLNK |
|---|---|
| Alternative Names | B-cell linker protein LY57 SLP-65 SLP65 |
| Molecular Weight(MW) | 50 kDa |
| Tissue Specificity | The antibody detects endogenous level of BLNK only when phosphorylated at tyrosine 96. |
| Entrez Gene | 29760 |
|---|---|
| Protein Accession | NM_001114094.1 |
Application
Western blot analysis of extracts from COLO205 tissue using BLNK (Phospho-Tyr96) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
