WB
Figure. Western Blot; Sample: Recombinant BLNK, Human.WB
Western Blot; Sample: Mouse Spleen lysate;IHC
Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue.Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human BLNK Polyclonal Antibody, filled red histogram) or Isotype control antibody (filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
| Product Name | Polyclonal Antibody to B-Cell Linker Protein (BLNK) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant BLNK (Ser65~Thr314) expressed in E.coli |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:0.01-2µg/mL FC:20µg/mL IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Symbol | BLNK |
|---|---|
| Gene Synonyms | bca AGM4 BASH LY57 SLP65 BLNK-S SLP-65 |
| Gene Full Name | B cell linker |
| Gene Summary | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] |
| Alternative Names | BASH BLNK-s Ly57 SLP65 B-Cell Adapter Containing A SH2 Domain Protein Cytoplasmic adapter protein Src homology 2 domain-containing leukocyte protein of 65 kDa |
| Molecular Weight(MW) | 31 kDa |
WB
Figure. Western Blot; Sample: Recombinant BLNK, Human.
WB
Western Blot; Sample: Mouse Spleen lysate;
IHC
Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue.
Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human BLNK Polyclonal Antibody, filled red histogram) or Isotype control antibody (filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
| Application Notes | WB:0.01-2µg/mL FC:20µg/mL IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. |
| Storage Buffer | Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol. |
