Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).Application
BLOC1S2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).| Product Name | BLOC1S2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BLOC1S2 is a component of the ubiquitously expressed BLOC1 multisubunit protein complex. BLOC1 is required for normal biogenesis of specialized organelles of the endosomal-lysosomal system, such as melanosomes and platelet dense granules (Starcevic and Dell'Angelica, 2004 [PubMed 15102850]).1) Rodriguez-Fernandez, I.A., et al. J. Inherit. Metab. Dis. 32(2):190-203(2009) |
| Immunogen | This BLOC1S2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the Central region of human BLOC1S2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BLOC1S2 |
|---|---|
| Alternative Names | Biogenesis of lysosome-related organelles complex 1 subunit 2 BLOC-1 subunit 2 Centrosome-associated protein BLOC1S2 BLOS2 CEAP |
| Molecular Weight(MW) | 16 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).
Application
BLOC1S2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in HepG2 cell line lysates (15ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
