Application
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Bloom Syndrome (phospho Thr99) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).1) Ellis N.A., Cell 83:655-666(1995). |
| Immunogen | Bloom Syndrome (phospho Thr99) antibody was raised against a peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p)-Q-R) derived from Human Bloom Syndrome. |
| Modification | p-Thr99 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BLM |
|---|---|
| Alternative Names | RECQ2 RECQL3 type 2 EC 3.6.1 RecQ protein-like 3 |
| Molecular Weight(MW) | 159 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99. |
| Entrez Gene | 641 |
|---|---|
| Protein Accession | NP_000048.1 |
Application
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
