Application
Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 μg/mL.Application
Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of Bmf in HeLa cells with Bmf antibody at 5 μg/mL.| Product Name | Bmf Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Bmf Antibody: Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.1) Puthalakath H, Villunger A, O'Reilly LA, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 2001; 293:1829-32. |
| Immunogen | Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf. The immunogen is located within the first 50 amino acids of Bmf. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMF |
|---|---|
| Alternative Names | Bmf Antibody: Bcl-2-modifying factor |
| Molecular Weight(MW) | Predicted: 20 kDa Observed: 22 kDa |
| Sequence Similarities | 4) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 μg/mL.
Application
Western blot analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of Bmf in HeLa cells with Bmf antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Bmf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bmf Antibody is supplied in PBS containing 0.02% sodium azide. |
