Application
Immunocytochemistry of BMI-1 in K562 cells with BMI-1 antibody at 10 μg/mL.Application
Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 μg/mL.| Product Name | BMI-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMI-1 Antibody: PCGF4, RNF51, FLVI2/BMI1, PCGF4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4 |
| Product description | BMI-1 Antibody: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.1) Alkema MJ, Wiegant J, Raap AK, et al. Characterization and chromosomal localization of the human proto-oncogene BMI-1. Hum. Mol. Genet. 1993; 2:1597-603. |
| Immunogen | BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1. The immunogen is located within amino acids 140 - 190 of BMI-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMI1 |
|---|---|
| Alternative Names | BMI-1 Antibody: PCGF4 RNF51 FLVI2/BMI1 PCGF4 Polycomb complex protein BMI-1 Polycomb group RING finger protein 4 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of BMI-1 in K562 cells with BMI-1 antibody at 10 μg/mL.
Application
Western blot analysis of BMI-1 in K562 cell lysate with BMI-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of BMI-1 in K562 cells with BMI-1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BMI-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
