Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of BMI1 Antibody antibodyin 293 cell line lysates (35ug/lane)| Product Name | BMI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, BMI1, PCGF4, RNF51 |
| Product description | BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.1) Smith, K.S., et al., Mol. Cell 12(2):393-400 (2003). Xia, Z.B., et al., Proc. Natl. Acad. Sci. U.S.A. 100(14):8342-8347 (2003). Itahana, K., et al., Mol. Cell. Biol. 23(1):389-401 (2003). Dimri, G.P., et al., Cancer Res. 62(16):4736-4745 (2002). Bardos, J.I., et al., J. Biol. Chem. 275(37):28785-28792 (2000). |
| Immunogen | This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMI1 |
|---|---|
| Alternative Names | Polycomb complex protein BMI-1 Polycomb group RING finger protein 4 RING finger protein 51 BMI1 PCGF4 RNF51 |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with BMI1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of BMI1 Antibody antibodyin 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
