Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BMP-2 (XP-5111Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2.| Product Name | BMP-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BDA2, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A, BMP-2 |
| Product description | BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBMP-2 (human BMP-2). |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ELISA: To detect hBMP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | HumanMouseRhesus Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMP2 |
|---|---|
| Alternative Names | BDA2 BMP2A Bone morphogenetic protein 2 Bone morphogenetic protein 2A BMP-2 |
| Molecular Weight(MW) | 45KD |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hBMP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human BMP-2 (XP-5111Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2.| Application Notes | ELISA: To detect hBMP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBMP-2. Western Blot: To detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | BMP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
