Application
This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.| Product Name | BMP-4 Antibody [3H2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix. |
| Immunogen | BMP-4 antibody is produced in mice using highly pure (>98%) recombinant human BMP-4 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BMP4 |
|---|---|
| Alternative Names | Bone morphogenetic protein BMP2B DVR4 Bone morphogenetic protein 4 Bone morphogenetic protein 2B BMP-4 ZYME OFC11 BMP2B1 MCOPS6 |
Application
This antibody stained HepG2 cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hBMP-4 (5.0 ng/mL), a concentration of 0.4-0.6 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2.0-4.0 ng/lane, under non-reducing conditions. |
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| Form | Liquid |
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| Storage Instructions | BMP-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
