Application
PSMA5 antibody detects PSMA5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. PSMA5 antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Proteasome 20S alpha 5 antibody at 1:100 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S alpha5 antibody at 1:200 dilution.Application
PSMA5 antibody detects PSMA5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA5 antibody diluted by 1:1000.| Product Name | PSMA5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMA5 (proteasome (prosome, macropain) subunit, alpha type, 5)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PSMA5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (97%), Xenopus laevis (98%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ42315 antibody MGC117302 antibody MGC125802 antibody MGC125803 antibody MGC125804 antibody PSC5 antibody ZETA antibody PSMA5 antibody proteasome alpha 5 subunit antibody macropain zeta chain antibody proteasome zeta chain antibody multicataly |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
PSMA5 antibody detects PSMA5 protein at cytoplasm and nucleus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. PSMA5 antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Proteasome 20S alpha 5 antibody at 1:100 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Proteasome 20S alpha5 antibody at 1:200 dilution.
Application
PSMA5 antibody detects PSMA5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PSMA5 antibody diluted by 1:1000.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
