Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | BOLL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein boule-like, BOLL, BOULE |
| Product description | BOLL belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition.1) Chen, P., et al. J. Androl. 31(2):163-168(2010) |
| Immunogen | This BOLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human BOLL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BOLL |
|---|---|
| Alternative Names | Protein boule-like BOLL BOULE |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
