BOP1 is the component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.1) Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BOP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
BOP1 antibody can be used for detection of BOP1 by ELISA at 1:62500. BOP1 antibody can be used for detection of BOP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
BOP1 antibody can be used for detection of BOP1 by ELISA at 1:62500. BOP1 antibody can be used for detection of BOP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store BOP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.