Application
Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 μg/mL.Application
Immunofluorescence of BRAL1 in Human Brain cells with BRAL1 antibody at 20 μg/mL.Application
Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | BRAL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRAL1 Antibody: BRAL1, Hyaluronan and proteoglycan link protein 2, Brain link protein 1 |
| Product description | BRAL1 Antibody: BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.1) Spicer AP, Joo A, and Bowling Jr. RA. A hyaluronan binding link protein gene family whose members are physically linked adjacent to chrondroitin sulfate proteoglycan core protein genes. J. Biol. Chem.2003; 278:21083-91. |
| Immunogen | BRAL1 antibody was raised against a 12 amino acid synthetic peptide from near the center of human BRAL1. The immunogen is located within amino acids 130 - 180 of BRAL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HAPLN2 |
|---|---|
| Alternative Names | BRAL1 Antibody: BRAL1 Hyaluronan and proteoglycan link protein 2 Brain link protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BRAL1 in Human Brain cells with BRAL1 antibody at 20 μg/mL.
Application
Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BRAL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BRAL1 Antibody is supplied in PBS containing 0.02% sodium azide. |
