Application
PSMB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PSMB1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PSMB1 Antibody (C-term) in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35ug/lane). PSMB1 (arrow) was detected using the purified Pab.| Product Name | PSMB1 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PSMB1 (C-term)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 2S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 2S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 212~241 amino acids from the C-terminal region of human PSMB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PSMB1 PSC5 PSMB1 Proteasome subunit beta type-1 Proteasome component C5 Proteasome gamma chain Macropain subunit C5 Proteasome subunit beta type1 Multicatalytic endopeptidase complex subunit C5 Proteasome subunit beta type 1 |
|---|---|
| Target | PSMB1 |
| Molecular Weight(MW) | 26.489 kDa (note) |
Application
PSMB1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PSMB1 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PSMB1 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PSMB1 Antibody (C-term) in mouse NIH-3T3 cell line(lane 1) and mouse bladder tissue(lane 2) lysates (35ug/lane). PSMB1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
