Application
Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | BRCA2 (phospho Ser3291) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRCC2, BRCA2, subunit 2, BRCA1/BRCA2-containing complex |
| Product description | Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures.1) Wooster R., Nature 378:789-792(1995). |
| Immunogen | BRCA2 (phospho Ser3291) antibody was raised against a peptide sequence around phosphorylation site of Serine 3291 (F-V-S (p)-P-A) derived from Human BRCA2. |
| Modification | p-Ser3291 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA2 |
|---|---|
| Alternative Names | BRCC2 BRCA2 subunit 2 BRCA1/BRCA2-containing complex |
| Molecular Weight(MW) | >350 kDa |
| Tissue Specificity | The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291. |
| Entrez Gene | 675 |
|---|---|
| Protein Accession | NP_000050.2 |
Application
Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
