Application
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 μg/mL.Application
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 μg/mL.Application
Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | BRCC36 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRCC36 Antibody: C6.1A, BRCC36, CXorf53, C6.1A, Lys-63-specific deubiquitinase BRCC36, BRCA1-A complex subunit BRCC36 |
| Product description | BRCC36 Antibody: Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.1) Dong Y, Hakimi MA, Chen X, et al. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalsome-like subunit and its role in DNA repair. Mol. Cell2003; 12:1087-99. |
| Immunogen | BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCC3 |
|---|---|
| Alternative Names | BRCC36 Antibody: C6.1A BRCC36 CXorf53 C6.1A Lys-63-specific deubiquitinase BRCC36 BRCA1-A complex subunit BRCC36 |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 35 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 μg/mL.
Application
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 μg/mL.
Application
Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | BRCC36 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BRCC36 Antibody is supplied in PBS containing 0.02% sodium azide. |
