Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | BRD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRD7, BP75, CELTIX1, NAG4 |
| Product description | BRD7 is an activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. It induces dephosphorylation of GSK3B at 'Tyr-216'.1) Zhou, J., (2004) J. Cell. Physiol. 200 (1), 89-98. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BRD7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| BRD7 antibody can be used for detection of BRD7 by ELISA at 1:312500. BRD7 antibody can be used for detection of BRD7 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRD7 |
|---|---|
| Alternative Names | BRD7 BP75 CELTIX1 NAG4 |
| Molecular Weight(MW) | 74 kDa |
Application
Antibody used in WB on Jurkat 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | BRD7 antibody can be used for detection of BRD7 by ELISA at 1:312500. BRD7 antibody can be used for detection of BRD7 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store BRD7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
