Application
Western blot analysis of extracts of various cell lines, using PSMB5 antibody.Application
Immunohistochemistry of paraffin-embedded human stomach using PSMB5 antibody at dilution of 1:200 (x400 lens).Application
Immunofluorescence analysis of U2OS cell using PSMB5 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of MCF-7 cell using PSMB5 antibody. Blue: DAPI for nuclear staining.| Product Name | PSMB5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PSMB5The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Immunogen | Recombinant protein of human PSMB5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | proteasome subunit beta 5 X LMPX MB1 |
|---|---|
| Target | PSMB5 |
Application
Western blot analysis of extracts of various cell lines, using PSMB5 antibody.
Application
Immunohistochemistry of paraffin-embedded human stomach using PSMB5 antibody at dilution of 1:200 (x400 lens).
Application
Immunofluorescence analysis of U2OS cell using PSMB5 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of MCF-7 cell using PSMB5 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
